F-013. In-vitro maturation of oocytes from domestic species

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Reactive oxygen species generation and use of antioxidants during in vitro maturation of oocytes: a Review

In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment and preservation. However, standard in vitro culture conditions usually increase reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic developmental competence. It is well-known that higher than physiological levels of ROS triggers granulosa ...

In vitro maturation and degeneration of domestic cat oocytes collected from ovaries stored at various temperatures

Cat oocytes have the unique ability to mature in vitro after temporary storage at 4°C which can provide opportunities to rescue oocytes from the ovaries of endangered felids after sudden death or medical ovariohysterectomy. It has been demonstrated that factors such as season, culture conditions and morphological quality of oocytes influence the meiotic competence of domestic cat oocytes. In th...

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

P-87: High Fat Diet Affects In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes

Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Mate...